Confocal laser scanning microscopy is a method for obtaining high-resolution optical images with depth selectivity. It is basically an optical imaging method used to increase optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. Confocal laser scanning microscope has the capability to acquire in-focus images from selected depths. It enables the reconstruction of three-dimensional structures from the obtained images by collecting sets of images at different depths (a process known as optical sectioning) within a thick object. Confocal laser scanning microscopes require very good optics, one or more lasers, an integrated beam scanning – image acquisition and image processing system. Confocal microscopy allows accurate and non-destructive optical sectioning in a plane perpendicular or parallel to the optical axis of the microscope. This method has a lot of demand in the sci...